Identification and Growth Inhibitory Activity of the Chemical Constituents from Imperata Cylindrica Aerial Part Ethyl Acetate Extract

摘要

Imperata cylindrica (L.) Raeusch. (IMP) aerial part ethyl acetate extract has anti-proliferative,pro-apoptotic, and pro-oxidative effects towards colorectal cancer in vitro. The chemical constituentsof IMP aerial part ethyl acetate extract were isolated using high-performance liquid chromatography(HPLC) and identified with tandem mass spectrometry (ESI-MS/MS) in combination withultraviolet-visible spectrophotometry and 400 MHz NMR. The growth inhibitory effects of eachidentified component on BT-549 (breast) and HT-29 (colon) cancer cell lines were evaluated after48/72 h treatment by MTT assay. Four isolated compounds were identified as trans-p-Coumaric acid(1); 2-Methoxyestrone (2); 11, 16-Dihydroxypregn-4-ene-3, 20-dione (3); and Tricin (4). Compounds(2), (3), and (4) exhibited considerable growth inhibitory activities against BT-549 and HT-29 cancercell lines. Compounds (2), (3), and (4) are potential candidates for novel anti-cancer agents againstbreast and colorectal cancers.