The Identification of Cryptosporidium Species by PCR-RFLP Analysis of the 18s rRNA Gene

摘要

Background: Cryptosporidium is an important protozoa that cause diarrheal illness in humans and animals.In immuoncompetent individuals,infection is usually self-limiting,but in immunocompromised patients infections which can be life threatening may develop. Cryptosporidium may account for 10 to 20% of the cases of diarrhea in immunocompromised patients living in developed countries and as much as 50% in underprivileged countries. Different strains of Cryptosporidium have been reported and it is belived that strain characteristics are an important factor to be cosidered is strategic planning for the control of cryptosporidiosis disease.In this study human and animal isolates of Cryptosporidium ooccysts were examined by PCR-RFLP for identifying strain variation in isfahan.