PATHOGEN IDENTIFICATION ON THE BASIS OF A 16S OR 18S RRNA MICROARRAY

摘要

Disclosed is a method for identification of microbial pathogens of bloodstream infections in a blood sample, comprising the steps of: a) providing a blood sample, b) lysing the microbial pathogens and performing a nucleic acid amplification reaction on the encoding 16S or 18S rRNA microbial DNA wherein or whereafter the amplified nucleic acids are labeled, c) contacting the labeled amplified nucleic acids from step b) with a microarray comprising immobilized probes for encoding 16S or 18S rRNA from microbial pathogens of bloodstream infections, microbial DNA on defined areas on the surface of microarrays, d) detecting the binding of one or more kind (s) of the labeled amplified nucleic acids to a probe by detecting a labeled amplified nucleic acid which is specifically bound to the microarray, ande) identifying a microbial pathogen of bloodstream infections in the blood sample by correlating the detected binding of the labeled amplified nucleic acids with the zoning of the immobilized probes for 16S or 18S rRNA from microbial pathogens of bloodstream infections encoding microbial DNA.