A method for increasing viral detection in human and wildlife samples with low nucleic acid content

摘要

Background: Detection of pathogens in the CSF of viral encephalitis (VE) patients and in excreta from wildlife is often limited by insufficient nucleic acid in these samples. This may explain why half of VE patients had etiologies identified and shot gun sequencing using high throughput sequencer (such as Roche 454 junior) is often necessary for these, as well as wildlife, samples. Yet outcomes from such diagnostic approaches are not always successful.