Metabolic Flux Analysis of hybridoma cells: underdetermined network and influence of batch and perfusion operating modes.

摘要

In this work, metabolic flux analysis (MFA) is applied to determine the flux distribution within a metabolic network representing the metabolism of a hybridoma cell line. Even though a relatively large set of extracellular measurements are available, mass balancing leads to an underdetermined system and a unique solution cannot be computed. To tackle this problem, a convex analysis approach is therefore used to compute the metabolic fluxes as positive bounded intervals, with the help of the toolbox METATOOL. Our main objective is to investigate the influence of the number of extracellular measurements and the culture operating mode, i.e., batch or perfusion, on the flux distribution. Our study is based on sets of experimental data from hybridoma HB58 cell batch/perfusion cultures.