design of whole cells using a real-time analysis of metabolic flux

摘要

A method for the whole - cell - engineering of new or modified phenotypes by the use of a metabolic real time - flow analysis, wherein said method comprising the steps of: (a) production of a modified cell by modifying the genetic composition of a cell; (b) cultivating the modified cell, in order to provide a plurality of modified to produce cells; (c) measuring of at least one metabolic parameters of the cell by monitoring the cell culture of step (b) in real time; and (d) analyzing the data from step (c), in order to determine whether the measured parameters of a comparable measurement in an unmodified cell under similar conditions, in order to obtain a constructed phenotype in the cell with metabolic real time - flow analysis to identify.