Regulatable Vectors for Environmental Gene Expression in Alphaproteobacteria

摘要

Two expression vectors utilizing the inducible taurine promoter ( tauAp ) were developed. Plasmid pLMB51 is a stable low-copy vector enabling expression in the environment and in planta . The higher copy number pLMB509 enables BD restriction-independent cloning, expression, and purification of polyhistidine-tagged proteins.