Effects of Minerals on Resistance of Bacillus subtilis Spores to Heat and Hydrostatic Pressure

摘要

Among Bacillus subtilis IFO13722 spores sporulated at 30, 37, and 44°C, those sporulated at 30°C had the highest resistance to treatments with high hydrostatic pressure (100 to 300 MPa, 55°C, 30 min). Pressure resistance increased after demineralization of the spores and decreased after remineralization of the spores with Ca2+ or Mg2+, whereas the resistance did not change when spores were remineralized with Mn2+ or K+, suggesting that former two divalent ions were involved in the activation of cortex-lytic enzymes during germination.