Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries

Jeppe L. Nielsen; Andreas Schramm; Anne E. Bernhard; Gerrit J. van den Engh; David A. Stahl

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Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries

机译：流式细胞术辅助从复杂的16S rRNA基因文库克隆特定序列的母题

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A flow cytometry method was developed for rapid screening and recovery of cloned DNA containing common sequence motifs. This approach, termed fluorescence-activated cell sorting-assisted cloning, was used to recover sequences affiliated with a unique lineage within the Bacteroidetes not abundant in a clone library of environmental 16S rRNA genes.

机译：开发了一种流式细胞术方法，用于快速筛选和回收含有常见序列基序的克隆DNA。这种被称为荧光激活细胞分选辅助克隆的方法，被用于回收在环境16S rRNA基因克隆文库中并不丰富的拟杆菌内的独特谱系的序列。

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《Applied and Environmental Microbiology》 |2004年第12期|共5页
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Jeppe L. Nielsen; Andreas Schramm; Anne E. Bernhard; Gerrit J. van den Engh; David A. Stahl;
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中图分类植物学;
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1. Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries [J] . Jeppe L. Nielsen, Andreas Schramm, Anne E. Bernhard, Applied and Environmental Microbiology . 2004,第12期

机译：流式细胞术辅助从复杂的16S rRNA基因文库克隆特定序列的母题
2. Diversity of Microflora in Colonic Mucus from Severe Ulcerative Colitis Patients Analyzed by Terminal Restriction Fragment Length Polymorphism and Clone Libraries of Bacterial 16S rRNA Gene Sequences [J] . I-Nung Huang, Yuri Sato, Mitsuo Sakamoto, Advances in Microbiology . 2014,第13期

机译：通过终端限制性片段长度多态性和细菌16S rRNA基因序列的克隆库分析严重溃疡性结肠炎患者结肠黏液中的菌群多样性。
3. The optimal duration of antimicrobial therapy for lower respiratory tract infection in patients with neuromuscular disorders based on a clone library analysis of the bacterial 16S rRNA gene sequence [J] . Masaru Kawamura, Takayuki Hoshina, Masato Ogawa, International journal of infectious diseases : . 2020,第3S1期

机译：基于细菌16S rRNA基因序列克隆文库分析的神经肌肉病患者患者抗菌治疗抗菌治疗的最佳持续时间
4. GENETIC DIVERSITY IN HEADWATER-SPECIFIC MAYFLIES BASED ON THE MITOCHONDRIAL 16S RRNA GENE SEQUENCES [C] . Koji Tojo International Symposium on Plecoptera . 2008

机译：基于线粒体16S rRNA基因序列的基于线粒体特异性mayflies的遗传多样性
5. Development of a 16S rRNA gene sequence as a biomarker specific to the gastrointestinal tract and feces of swine based on suppressive subtractive hybridization. [D] . Sayre, Autumn. 2013

机译：基于抑制性消减杂交技术开发了一种16S rRNA基因序列，作为猪胃肠道和粪便的生物标记物。
6. Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries [O] . Jeppe L. Nielsen, Andreas Schramm, Anne E. Bernhard, 2004

机译：流式细胞术辅助从复杂的16S rRNA基因文库克隆特定序列的母题
7. Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries [O] . Nielsen, Jeppe L., Schramm, Andreas, Bernhard, Anne E., 2004

机译：流式细胞术辅助从复杂的16S rRNA基因文库克隆特定序列的母题

Flow Cytometry-Assisted Cloning of Specific Sequence Motifs from Complex 16S rRNA Gene Libraries

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