首页> 外文期刊>Applied and Environmental Microbiology >Differential Expression of Pine and Cronartium quercuum f. sp. fusiforme Genes in Fusiform Rust Galls
【24h】

Differential Expression of Pine and Cronartium quercuum f. sp. fusiforme Genes in Fusiform Rust Galls

机译:松木和克伦纳豆的差异表达f。 sp。梭形锈Gall中的梭形基因

获取原文
           

摘要

Cronartium quercuum f. sp. fusiforme is the causative agent of fusiform rust disease of southern pines in the United States. This disease is characterized by the formation of woody branch and stem galls. Differential display was used to identify pine genes whose expression is altered by C. quercuum f. sp. fusiforme infection and to identify C. quercuum f. sp. fusiforme genes that are expressed in fusiform rust galls. Six pine cDNAs that appeared to be differentially expressed in galled and healthy stems and 13 C. quercuum f. sp. fusiforme cDNAs expressed in galled tissues were identified. A probe that hybridizes specifically to C. quercuum f. sp. fusiforme 18S rRNA was used to estimate that 14% of the total RNA in fusiform rust galls was from C. quercuum f. sp. fusiforme. This finding was used to calibrate gene expression levels in galls when comparing them to expression levels in uninfected pines or in isolated C. quercuum f. sp. fusiforme cultures. According to Northern analysis and reverse transcriptase PCR analysis, all six of the pine clones were expressed at lower levels in galls than in healthy tissues. Seven of the nine C. quercuum f. sp. fusiforme clones that were assayed were expressed at higher levels in galls than in axenic culture. A number of the cDNAs encode proteins that are similar to those that play roles in plant development, plant defense, or fungal stress responses.
机译:槲皮草f。 sp。 fusiforme是美国南部松树的fusiform锈病的病原。该病的特征是木质分支和茎gall形成。使用差异展示来鉴定其表达被槲皮梭菌改变的松树基因。 sp。梭状杆菌感染和鉴定栎形梭菌f。 sp。在梭形锈gall中表达的梭形基因。六个松树cDNA在胆汁和健康茎和13 C. quercuum f。中似乎差异表达。 sp。鉴定了在伤口组织中表达的梭形cDNA。一种特异性杂交至que.curcuum f。的探针。 sp。梭形虫18S rRNA用于估计梭形锈虫胆中总RNA的14%来自槲皮液。 sp。梭形目。当将其与未感染的松树或分离的槲皮C.中的表达水平进行比较时,此发现可用于校准胆汁中的基因表达水平。 sp。梭形文化。根据Northern分析和逆转录PCR分析,所有六个松树克隆在胆汁中的表达均低于健康组织。九个槲寄生中的七个f。 sp。被检出的梭形克隆在胆汁中的表达水平高于在轴突培养物中的表达水平。许多cDNA编码的蛋白质与在植物发育,植物防御或真菌胁迫反应中起作用的蛋白质相似。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号