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Restriction fragment length polymorphism evidence for genetic homology within a pathovar of Pseudomonas syringae.

机译:限制性假单胞菌丁香假单胞菌内遗传同源性的限制性片段长度多态性证据。

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Pseudomonas syringae pv. phaseolicola NPS3121 hrp sequences were used as hybridization probes in a restriction fragment length polymorphism (RFLP) analysis of 24 P. syringae pv. tabaci strains as a means to evaluate the genetic and taxonomic relationship of pathovars of P. syringae. Southern blot analyses of genomic restriction digests, with hrpA-S sequences as hybridization probes, and restriction analyses of PCR-amplified DNA of regions within hrpD were conducted. The resulting RFLP patterns were uniform for 23 of the 24 isolates tested, with strain BR2R having a unique pattern. BR2R is a pathogen of bean which was classified as pathovar tabaci because of its ability to produce tabtoxin, but unlike the other 23 tabaci strains in this study, it does not incite disease symptoms on tobacco. When a DNA fragment containing hrpM sequences was used as a hybridization probe, the tabaci isolates could be divided into three groups on the basis of the RFLP patterns : BR2R, Pt11528R and Pt113R, and the remaining strains. For all of the above analyses, BR2R shared identical RFLP patterns with P. syringae pv. phaseolicola NPS3121, also a bean pathogen which does not cause disease on tobacco. However, BR2R AND NPS3121 could be differentiated from each other on the basis of the RFLP patterns from restriction analysis of PCR-amplified DNA of argF, while the remaining tabaci strains had a third pattern. These studies indicate that hrp genes and argF are conserved in strains of P. syringae pathogenic to tobacco, suggesting that P. syringae strains pathogenic to specific hosts may have a high level of genetic similarity. We believe that these analyses have shown that distinct identifiable genetic differences may be correlated with host range and suggest that such information may be useful for assigning pathovar designations.
机译:丁香假单胞菌PV。菜豆属NPS3121 hrp序列在24个丁香假单胞菌pv的限制性片段长度多态性(RFLP)分析中用作杂交探针。烟粉虱菌株作为评估丁香假单胞菌病原菌遗传和分类学关系的一种手段。使用hrpA-S序列作为杂交探针进行基因组限制性消化的Southern印迹分析,并对hrpD内区域的PCR扩增DNA进行限制性分析。对于测试的24个分离株中的23个,所得的RFLP模式是一致的,菌株BR2R具有独特的模式。 BR2R是一种大豆病原体,由于其产生毒素的能力而被归类为病原菌,但与本研究中的其他23种烟曲霉菌株不同,它不会在烟草上引起疾病症状。当将包含hrpM序列的DNA片段用作杂交探针时,根据RFLP模式,可将烟粉分离株分为三类:BR2R,Pt11528R和Pt113R,以及其余的菌株。对于上述所有分析,BR2R与丁香假单胞菌pv共享相同的RFLP模式。菜豆NPS3121,也是一种大豆病原体,不会引起烟草疾病。但是,可以通过基于argF的PCR扩增DNA的限制性酶切分析的RFLP模式,将BR2R和NPS3121彼此区分开,而其余的烟粉虱菌株则具有第三种模式。这些研究表明,hrp基因和argF在对烟草致病的丁香假单胞菌菌株中是保守的,这表明对特定宿主致病的丁香假单胞菌菌株可能具有高水平的遗传相似性。我们认为,这些分析表明,不同的可识别遗传差异可能与寄主范围有关,并表明此类信息可能对分配病原菌名称有用。

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