Electroporation-induced transformation of intact cells of Clostridium perfringens 3624A with plasmids pAMB1 and pHR106 resulted in 3.8 X 10(-5) and 4.2 X 10(-4) transformants per viable cell, respectively. With respect to shuttle plasmid pHR106, these values represent a greater than 100-fold increase in transformation frequency when compared with the values reported with polyethylene glycol-induced L-phase variants.
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机译:用质粒pAMB1和pHR106电穿孔诱导产气荚膜梭菌3624A完整细胞的转化,每个活细胞分别产生3.8 X 10(-5)和4.2 X 10(-4)转化子。关于穿梭质粒pHR106,当与聚乙二醇诱导的L相变体报道的值相比时,这些值表示转化频率增加了大于100倍。
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