A Fluorometric Determination of Trypsin-Like Amidase Activity and Activity of Trypsin Inhibitors in Serum

摘要

The activity of crystalline trypsin and the trypsin-like amidase activity and the activity of trypsin inhibitors in serum were measured fluorometically, with α-benzoyl-L-arginine-β-naphthylamide (BANA) as substrate. The method is simpler and more sensitive than the colorimetric method based on the Bratton-Marshall reaction. Serum of pancreatectomized dogs hydrolyzed BANA at a significant rate, which suggests that serum amidases other than trypsin are active in this determination.