The chromatographic behaviors of nucleotides (inosine 5`-monophosphate, uridine 5`-monophosphate, guanosine 5`-monophosphate, and thymine monophosphate disodium salts) on a C18 column were studied with different types of ionic liquids (ILs) as additives for the mobile phase in reversed-phase liquid chromatography (RPLC). Three ILs, 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIm][BF4]), 1-ethyl-3-methylimidazolium tetrafluoroborate ([EMIm][BF4]), and 1-ethyl-3-methylimidazolium methylsulfate ([EMIm][MS]), were used. Eluents were composed of water and methanol (90/10%, vol) with the addition of 0.5-13.0 mM of ILs. The effects of the concentration of ILs on retention and separation were investigated and discussed. The results showed that the addition of ILs affects the retention and resolution of the tested compounds. Use of 13.0 mM of [BMIm][BF4] as the eluent modifier resulted in a baseline separation of nucleotides without requiring gradient elution. This study demonstrates that ILs can be potentially applied as a mobile phase modifier in RPLC.
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机译：使用不同类型的离子液体（ILs）作为移动添加剂，研究了C18色谱柱上核苷酸（肌苷5`-单磷酸，尿苷5`-单磷酸，鸟苷5`-单磷酸和胸腺嘧啶一磷酸二钠盐）的色谱行为反相液相色谱（RPLC）三个IL，1-丁基-3-甲基咪唑四氟硼酸酯（[BMIm] [BF4]），1-乙基-3-甲基咪唑四氟硼酸酯（[EMIm] [BF4]）和1-乙基-3-甲基咪唑甲基硫酸甲酯（[EMIm] [MS]）。洗脱液由水和甲醇（90/10％，体积）以及0.5-13.0 mM ILs组成。研究和讨论了IL浓度对保留和分离的影响。结果表明，IL的添加会影响被测化合物的保留和分离度。使用13.0 mM的[BMIm] [BF4]作为洗脱液修饰剂可导致核苷酸的基线分离，而无需梯度洗脱。这项研究表明IL可以潜在地用作RPLC中的流动相改性剂。
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