The Formation of Rope- and Pebbles-Type Aggregation from the Micro-End-to-End and -Side-by-Side Aggregates in Poly(L-proline) Solutions

摘要

Morphological studies in the micro-end-to-end (m-E-E) and micro-side-by-side (m-S-S) aggregations were conducted by using of scanning electron microscope (SEM) for the samples precipitated by heating of the end-products of the transition of Formケ (left-handed helix, three peptides per turn, 31) Form グ (right-handed helix, 3.3 peptides per turn, 103) in poly(L-proline) (PLP) in acetic acid(water)-propanol (1:9 v/v) solvent. The observed morphology for the solide state shows a rope (or super helical) type and pebbles type aggregate for the (m-E-E) and (m-S-S) aggregate respectively. The viscosities were also measured during the heat-precipitation in order to elucidate the process of formation of the rope- and pebbles-type aggregates. The result for the (m-E-E) aggregations exhibit two steps, i.e., at first, the viscosity increases with time (step 1), thereafter it decrease until attain the last value (step 2). But the (m-S-S) aggregations show only one step in the decreases in viscosity. On the bases of all experimental results it is possible to propose a reasonable mechanism for the formation of the two types of aggregates of the (m-E-E) and (m-S-S).