Effect of RAGE -429T / C and Gly82Ser Gene Polymorphism in Diabetic Retinopathy in General Hospital Mohammad Hoesin Palembang

摘要

The development of diabetic retinopathy varies in a number of populations, suggesting genetic factors contribute to its pathogenesis, compatible with the severity of hyperglycemia that occurs. One of the underlying theories is the increased glycosylation of enzymatic proteins that are irreversible to the formation of AGE. To know is there any effect of RAGE Genetic allele polymorphism in diabetics retinopathy in General Hospital Mohammad Hoesin Palembang. This study was an observational analytical study comparative study (case control study) on 90 subjects consisting of 30 people with type 2 diabetes with retinopathy, 30 people with DM without retinopathy and 30 normal control people who had a family relationship with case group. Frequency of RAGE -429T / C & Gly82Ser alelle gene is performed by PCR amplification and RFLP (Restriction Fragment Length Polymorphism) by using Alu1 enzyme. Frequency of C alleles is 20% from the DR group, 15% from the DNR, and 5% from control group. Odds Ratio was 7,85 with p = 0.028. Frequency of wild genotype alleles in the Gly82Ser RAGE gene was found 40% in DR, 53.33% in DNR, 70% in control group, whereas the mutant allele were 60% in DR, 46.67% in DNR, 30% in control group. Frequency of Gly allele is 70% in DR, 23% in DNR, 85% in control group and frequency of Ser DR allele (30%), RND (70%), 15% in control group, Chi Square test with p = 0.065, α=0.05, Odds Ratio 5,57, CI: 95%. There is the effect of variant RAGE -429T / C and Gly82Ser allele polymorphism in diabetic retinopathy patients.