Effect of Low Intensity Exercise toward Postsynaptic Density 95 Level and Spatial Memory in Male Swiss Webster Mice Induced by Immobilization Stress

摘要

Physical exercise has long been proven as a way to increase metabolic status, synaptic plasticity and protein regulation in order to maintain cognitive function and brain health. There has been a limited study focusing on the protective effect of physical exercise towards cognitive function during stress. This research is quasi experimental studies with post test only control group design, conducted in the animal house and molecular biology laboratory of Medical Faculty of Universitas Sriwijaya. As many as 32 white mice age 10 weeks old weighing 25-35 gr were divided into four groups. The first group was the control group, the second one was treated with immobilization stress 2 hours/daily for 21 days, the third group had been conditioned for 30 minute running at a speed of 11 m/min for 14 days, while the fourth one was treated with physical exercise after being exposure to immobilization stress. The post synaptic density (PSD) 95 level in hippocampus and serum cortisol were measured by using enzyme-linked immunosorbent assay, while spatial memory was assessed by using Morris Water Maze Test. Iimmobilization stress for 21 days showed significant elevation of serum cortisol as well as decrement of PSD 95 level and spatial memory compared to control group. Low intensity physical exercise showed significant elevation of PSD 95 level and spatial memory compared to control group. Therefore, low intensity physical exercise can prevent the decrement of PSD 95 level and spatial memory due to stress.