Assessment of long-term cultivated human precision-cut lung slices as an ex vivo system for evaluation of chronic cytotoxicity and functionality

摘要

Background Investigation of basic chronic inflammatory mechanisms and development of new therapeutics targeting the respiratory tract requires appropriate testing systems, including those to monitor long- persistence. Human precision-cut lung slices (PCLS) have been demonstrated to mimic the human respiratory tract and have potential of an alternative, ex-vivo system to replace or augment in-vitro testing and animal models. So far, most research on PCLS has been conducted for short cultivation periods (≤72?h), while analyses of slowly metabolized therapeutics require long-term survival of PCLS in culture. In the present study, we evaluated viability, physiology and structural integrity of PCLS cultured for up to 15?days. Methods PCLS were cultured for 15?days and various parameters were assessed at different time points. Results Structural integrity and viability of cultured PCLS remained constant for 15?days. Moreover, bronchoconstriction was inducible over the whole period of cultivation, though with decreased sensitivity (EC₅₀1d?=?4?×?10?8?M vs. EC₅₀15d?=?4?×?10?6?M) and reduced maximum of initial airway area (1d?=?0.5% vs. 15d?=?18.7%). In contrast, even though still clearly inducible compared to medium control, LPS-induced TNF-α secretion decreased significantly from day 1 to day 15 of culture. Conclusions Overall, though long-term cultivation of PCLS need further investigation for cytokine secretion, possibly on a cellular level, PCLS are feasible for bronchoconstriction studies and toxicity assays.