Dietary incorporation of whey protein isolate and galactooligosaccharides exhibits improvement in glucose homeostasis and insulin resistance in high fat diet fed mice

摘要

Background: This study was planned to investigate the effectiveness of the whey protein isolate (WPI) of high purity and a galactooligosaccharides (GOS) preparation on glucose homeostasis and insulin resistance in high fat diet (HFD) (45.47% energy from fat) fed conditions in C57BL/6J mice. Methods: Fasting blood glucose level, serum insulin, and glucagon-like peptide-1 (enzyme-linked immunosorbent assay) were measured; also, homeostasis model assessment of insulin resistance (HOMA-IR) was determined in different treatment groups. mRNA expression of gluconeogenesis genes in liver and small intestine tissues was analyzed by quantitative real time-polymerase chain reaction. Results: Dietary incorporation of WPI and GOS was observed to significantly resist ( P Conclusion: Both types of functional components exhibit potential to improve glucose homeostasis under HFD fed conditions. Resistance to HFD-induced hyperinsulinemia and HOMA-IR is also recorded .