This work describes two procedures based on residual base determination for the quantification of pefloxacin mesylate (PFM) in bulk drug and in pharmaceutical products. In the first method involving titrimetry, the drug solution is treated with a measured excess of sodium hydroxide followed by back titration of the residual base with hydrochloric acid using a phenol red-bromothymol blue mixed indicator. The second spectrophotometrie method involves treatment of a fixed amount of sodium hydroxide – phenol red mixture with varying amounts of the drug, and measuring the decrease in the absorbance of the dye at 560 nm. In the titrimetric method, a reaction stoichiometry of 1:1 was found in the quantification range of 4–20 mg of drug. The spectrophotometric method allows the determination of PFM in the 5–40 μg ml-1 range. The molar absorptivity is 5.91 x 103l mol-1 cm-1 and the Sandell sensitivity is 56.37 ng cm-2. The methods were applied successfully to the determination of PFM in pharmaceutical preparations.
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机译：这项工作描述了基于残留碱测定的两种程序，用于定量散装药物和药品中的甲磺酸培氟沙星（PFM）。在涉及滴定法的第一种方法中，先用测得的过量氢氧化钠处理药物溶液，然后使用酚红-溴百里酚蓝混合指示剂用盐酸反滴定残留的碱。第二种分光光度法包括用不同量的药物处理固定量的氢氧化钠-酚红混合物，并测量染料在560 nm处吸光度的降低。在滴定法中，在4–20 mg药物的定量范围内发现反应化学计量比为1：1。分光光度法可以测定5–40μgml-1范围内的PFM。摩尔吸收率是5.91 x 103l mol-1 cm-1，Sandell灵敏度是56.37 ng cm-2。该方法已成功应用于药物制剂中PFM的测定。
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