Preparation of Microcolumns Made of Glass-Lined Stainless Steel Tubing or Other Materials and Their Chromatographic Application

摘要

We have prepared microcolumns made of stainless steel tubing, glass-lined stainless steel tubing, and silica-lined stainless steel tubing and examined their performances comparatively. The length of columns was fixed as 30 cm, and the I.D.'s, either 1 mm or 0.5 mm. Octadecyl bonded silica paticles (5￥ì) were used as the stationary phases. p-Nitroaniline, N,N-dimethyl-o-nitro-p-toluidine,and ethylbenzene were used as the test solutes. The glass-lined or silica-lined stainless steel columns showed better performances than the plain stainless steel column did. The number of theoretical plates over 10,000 were obtained even for the 0.5 mm I.D. (30 cm long) column as long as the glass-lined stainless steel tubing was used.