Detecting Ureaplasma urealyticum among HIV-infected women with or without human papillomavirus using real-time PCR with the ANYPLEX? II STI-7 assay system

摘要

ObjectivesHuman immunodeficiency virus (HIV) infections increase the chances of women contracting human papillomavirus (HPV), the prime causative agent of cervical cancer. Additionally,Ureaplasma urealyticumis the most frequent pathogen prevailing in sexually transmitted diseases in HIV-infected women. The aim of this study was to determine the presence ofU.?urealyticumamong HIV-infected women with or without HPV in Ghana.MethodsDNA samples were extracted from cervico-vaginal swabs obtained from 96 HIV-infected women attending the Kumasi South Hospital.U.?urealyticumin the DNA samples was detected by real-time PCR with the Anyplex? II STI-7 detection assay. Microsoft Excel was used to analyze the data, and the Chi-square test was used to determine associations and dependence among the variables.ResultsAmong the study population, 93.75% (90/96) were positive for at least one pathogen.In total, 36.5% (35/96) of the women were infected byU.?urealyticum, with 30.21% (29/96) being co-infected with HPV. There was no significant association (95% CI,p?>?0.05) betweenU.?urealyticumand HPV status among the HIV-infected women.ConclusionU.?urealyticumand HPV are highly pathogenic, and their prevalence in this study reiterates the need for their routine screening in HIV-infected patients.