Establishment of plant regeneration of Michelia champaca L. through cell suspension culture technique

摘要

Michelia champaca?L. is a woody tree species, which has high economical value as a basic material for medicinal and fragrance products. However, there are several problems encountered in conventional propagation of?M. champaca,?which are generally low through seed germination, while vegetative layering technique produces limited planting materials. This experiment was conducted to establish high frequency?M. champaca?regeneration through cell suspension culture technique. Liquid Murashige and Skoog medium containing 2 mgL-1?α-naphthalene acetic acids induced high frequency of somatic embryos formation (47.67 ± 4.53 per ml). Meanwhile for germination of somatic embryos, the highest total number of somatic embryos germinated into plantlets obtained was 34% in hormone free Murashige and Skoog medium. Plant regeneration of?M. champaca?via cell suspension culture technique which was successfully established in this study could be used for mass production of planting materials of this tree.