Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae)

摘要

The present study was?conducted?to assess the antioxidant properties of ethanol, ethyl acetate, methanol and hexane extracts from leaves of?Pereskia bleo?using 2,2-diphenyl-1-picrylhydrazyl method? (DPPH assay), ferric reducing antioxidant power (FRAP assay) and β-Carotene-Linoleic acid methods. Total phenolic compounds (TPC) and flavonoids of the leaves of?P. bleo?were measured using Folin-Ciocalteu and HPLC methods respectively. Results of the present study showed that Ethyl acetate extract of?P. bleo?leaf exhibited significantly (p < 0.05) higher antioxidant activity as measured using DPPH free radical (IC50), FRAP and β-carotene-linoleic assays than that of hexane extract, methanol extract and ethanol extract.?Pereskia?bleo?leaf was also found to contain high amounts of bioactive compounds including total phenolic compounds (109.43 ±0.84 mg GAE/g), epicatechin (575± 0.04 mg/100 g), quercetin (110.94 ±0.12 mg/100 g), catechin (918 ± 0.01 mg/100 g) and myricetin (9.49±0.55 mg/100g) while the concentrations of α- tocopherol, β-carotene and lycopene were found to be higher (69±0.25, 51.97±1.18 mg/100g, 92.46±0.41 μg/g), respectively.