Alpha glucosidase inhibitory effect, anti-microbial activity and UPLC analysis of Rhus verniciflua under various extract conditions

摘要

In the present study, crude extract?of?Rhus verniciflua?stem was screened for its α-glucosidase inhibitory effects and anti-microbial activity using different extraction ratios of water to alcohol. Ultra performance liquid chromatography (UPLC) was used to quantify compounds (fustin, gallic acid, 3', 4', 7-trihydroxyflavone, and fisetin). Ethanolic extract showed higher α-glucosidase inhibitory effect than methanolic extract, whereas distilled water extract did not inhibit α-glucosidase at all. Especially, neat alcoholic (methanolic or ethanolic) extracts exhibited stronger inhibitory activities compared to their corresponding aqueous mixtures. Analysis of the UPLC chromatogram showed that fustin (from 28.5 to 151.7 mg/g extract) was the major compound of?R. verniciflua?stem. One-hundred percent ethanolic extracts produced inhibition zones against?Staphylococcus aureus?with the largest diameters (15 mm). The result from this study provides suitable support for the use of?R.?verniciflua?in the treatment of diabetes and for the control of infectious disease caused by?S. aureus.