Automation of Human IgG, Glucose, Lactate, and Oxygen Assays on Biomek NXP Span-8 Automation Workstation and PARADIGM Detection Platform

摘要

When optimizing cell culture media for therapeutic antibody production cell lines, it is very important to monitor parameters such as IgG concentration, glucose and lactate levels, and oxygen consumption in culture medium in an automated high-throughput manner to ensure the desirable growth conditions. In this report, assays for measuring human IgG, glucose, and lactate concentrations, and oxygen consumption were automated using the Biomek NXP Span-8 workstation, and analyzed on the PARADIGM Detection Platform. The human-IgG homogeneous time-resolved fluorescence (HTRF) (All trademarks are the property of their respective owners.) assay from Cisbio Inc. (Cisbio US, Bedford, MA) was automated in a 384-well plate format and detected on the PARADIGM with a HTRF Detection Cartridge. Glucose and lactate assays were automated in a 96-well plate format and detected on the PARADIGM with a Fluorescence Intensity Cartridge. Oxygen consumption was determined by the 96-well calibration-free OxoPlate (All trademarks are the property of their respective owners.) (PreSens Inc., PreSens - Precision Sensing GmbH, Regensburg, Germany) and detected by the PARADIGM with a MultiMode Detection Cartridge. The data for human IgG, glucose, lactate, and oxygen consumption assays automated on the Biomek NXP demonstrate the accuracy and precision obtained using the automated protocols on the PARADIGM Detection Platform, with the user-friendly and assay-specific cartridges for easy, quick, and accurate detection. Using this as a model, one would be able to adapt these approaches to high-throughput media optimization and cell monitoring.