High Speed Photography of Laser Induced Forward Transfer (LIFT) of Single and Double-layered Transfer Layers for Single Cell Transfer

摘要

Bioprinting technologies allow the construction of tissue-like structures from different cell populations.Fundamental research on the influence of the cell micro-environment requires printing of singlecells in specific patterns on a microscopic scale. Single cell printing of living cells has been performedby nozzle based techniques or micro-pipetting, and laser-induced forward transfer (LIFT)has turned out to enable a virtually contact-free printing process. In this work the LIFT process fromgelatine layers of 4% and 5% concentration is investigated using a high speed camera with 300,000fps. We analyze differences in the jetting behavior caused by differently prepared transfer layers andthe laser fluence. We assume that the velocity of a first, fast jet is a key parameter to control the viabilityof cells and precision of positioning. In comparison to a single gelatine layer a double-layerprepared from 5% gelatine coated with cell containing medium resulted in high cell survival rateand good precision.