Effect of Aidi injection combined with FOLFOX4 chemotherapy on tumor stem cell characteristics and antitumor immune response in patients with advanced colon cancer

摘要

Objective: To study the effect of Aidi injection combined with FOLFOX4 chemotherapy ontumor stem cell characteristics and antitumor immune response in patients with advancedcolon cancer. Methods: Patients with advanced colon cancer who received chemotherapyin our hospital between May 2013 and June 2016 were selected and randomly divided intothe combined chemotherapy group who received Aidi injection combined with FOLFOX4chemotherapy and the FOLFOX4 group who received FOLFOX4 chemotherapy alone. Afterchemotherapy, the serum was collected to determine the levels of tumor markers, tumorlesions were collected to determine the expression of tumor stem cell markers and immune cellmarkers, and peripheral blood mononuclear cells were collected to determine the expressionof immune cell markers. Results: After 2 and 4 cycles of treatment, serum CEA, CA199,CCSA-3 and CCSA-4 levels of combined chemotherapy group were significantly lower thanthose of FOLFOX4 group, and the mean fluorescence intensity of CD3, CD4, CD8, CD16and CD56 in peripheral blood mononuclear cells were significantly higher than those ofFOLFOX4 group; after four cycles of chemotherapy, CD133, Musashi-1, Piwil2, Nanog andSox-2 protein content in tumor lesions were significantly lower than those of FOLFOX4 group,and the mean fluorescence intensity of CD3, CD4, CD8, CD16 and CD56 were significantlyhigher than those of FOLFOX4 group. Conclusion: Aidi injection combined with FOLFOX4chemotherapy treatment of advanced colon cancer will help reduce the tumor load, inhibittumor stem cell characteristics and enhance antitumor immune response.