Effects of chondro-itinrase ABC combined with Jisuikang on neurological recovery and TGF-β1, HIF-1α and Nog-oNgR signaling pathways after spinal cord injury in rats

摘要

Objective: To explore the effects of chondroitinase ABC (Ch ABC) combined with spinalcord on neurological recovery and TGF-β1, HIF-1α and Nog-oNgR signaling pathways afterspinal cord injury in rats. Methods: Sixty rats were randomly divided into 4 groups: controlgroup, model group, Ch ABC group and Ch ABC+ spinal cord. A rat spinal cord injurymodel was established using the Allen's method. Spinal nerve function was assessed by BassoBeattie Bresnahan (score) and somatosensory evoked potential (sEP) assays. mRNA levelswere detected using RT-qPCR. Western blot was used to detect protein levels. Results: Aftermodeling, the BBB scores of the rats were significantly decreased. After the intervention, theBBB scores of the three groups were improved. The BBB scores of the Ch ABC+ spinal cordgroup were significantly higher than those of the Ch ABC group. After modeling, the SEP ofthe rats was significantly increased. After the intervention, the BBB scores of the three groupswere decreased. The BBB scores of the Ch ABC+ spinal cord group were significantly lowerthan those of the Ch ABC group. The TGF-β1 in the Ch ABC group and the Ch ABC+ spinalgroup was significantly higher than that in the model group, and the HIF-1α was significantlylower than the model group. The level of TGF-β1 in Ch ABC+ spinal cord group wassignificantly higher than that in Ch ABC group and HIF-1α was significantly lower than thatin Ch ABC group. After treatment, the expression levels of Nog-oNgR pathway in Ch ABCgroup and Ch ABC + spinal cord group were significantly lower than those in model group,and the expression levels of Nogo-A, NgR and LINGO-1 in Ch ABC+ spinal cord group weresignificant after intervention. Lower than Ch ABC group. Conclusion: ChABC combinedwith spinal cord has a stronger role in promoting the recovery of neurological function. Thecombination of spinal cord can further inhibit the level of HIF-1α and increase the level ofTGF-β1, and improve the prognosis to promote spinal healing. And the mechanism of actionof the combination may be related to its role in inhibiting the growth of neurons by the Nogo-NgR signaling pathway.