硫酸软骨素酶ABC联合超短波治疗对脊髓损伤大鼠的功能恢复及胶质瘢痕形成的影响

摘要

Objectives:To investigate the effects of ultrashortwave combined with chondritinase ABC (ChABC)on the functional recovery and glial scar formation factors such as glial fibrillaty acidic protein (GFAP)and chondroitin sulphate proteoglycans (CSPGs)after spinal cord injury (SCI).Method:Forty-two 8-week-old SD rats were ran-domly assigned to 3 groups:control group (including simple injury group and PBS solution injected group after inju-ry),ChABC group,ChABC combined with ultrashortwave treatment group (n=14 each).BBB score was evaluated at 1st day,1st week,2nd week,3rd week,and 4th week after SCI.SABC immunohistochemistry staining was used to observe the mean optical density (MOD)of GFAP and CSPGs at 2nd week and 4th week after SCI.Result:(1) BBB behavior scores in combination treatment group were significantly higher than in control group from 1st week to 4th week after SCI (P <0.05,0.01).BBB scores in ChABC treatment group were significantly higher than in con-trol group only at 4th week after injury (P <0.01).There was no difference between ChABC treatment group and combination treatment group (P >0.05).(2)As compared with the control group,the MOD of GFAP and CSPGs in the two treatment groups were both significantly reduced at 2nd week after injury (P < 0.01 ).There were no difference among the three groups in the MOD of GFAP and CSPGs at 4th week after injury.Conclusion:ChABC combined with ultrashortwave therapy can promote neurological function recovery after SCI and inhibit glial scar for-mation at 2nd week after injury,but the effects had not been sustained.So the concrete mechanism needs to be fur-ther studied.%目的：：本研究旨在观察超短波联合硫酸软骨素酶 ABC(ChABC)治疗对脊髓损伤术后功能恢复及与损伤后胶质瘢痕形成有关的因子如：胶质纤维酸性蛋白(GFAP)、硫酸软骨素蛋白聚糖(CSPGs)的影响。方法：42只8周龄 SD 大鼠随机分成3组,每组14只：对照组(包括8只单纯损伤组和6只损伤后注射 PBS 溶液组)、ChABC 组、ChABC 联合超短波治疗组。大鼠脊髓损伤后1d 及1周、2周、3周、4周做 BBB 神经行为学评分,应用免疫组织化学 SABC 法染色,分别于术后2周、4周检测脊髓组织中 GFAP、CSPGs 表达的平均光密度值差异。结果：BBB 评分比较：术后1周到4周,联合治疗组在各时间点的 BBB 评分均高于对照组(P <0.01,0.05),而 ChABC 组仅在术后第4周明显高于对照组(P <0.01)；2个治疗组间比较差异均无统计学意义。GFAP、CSPGs 免疫组化分析：与对照组相比,术后2周 ChABC 组和联合组的 GFAP、CSPGs 平均光密度度值均明显降低(P <0.01)。术后4周时, ChABC 组和联合组的 GFAP、CSPGs 平均光密度度值与对照组相比差异均无统计学意义(P >0.05)。结论：ChABC 联合超短波治疗能更好的促进脊髓损伤后的神经功能的恢复,并可在2周时抑制胶质瘢痕形成,但作用不持久,具体机制有待进一步研究。