Application of high-performance liquid chromatography–UV detection to quantification of clenbuterol in bovine liver samples

摘要

Abstract A method for the determination of clenbuterol (CLB) concentration in bovine liver by reversed-phase high-performance liquid chromatography (HPLC) with {UV} detection was developed. The sample was extracted with acetonitrile and isopropanol, followed by {HPLC} analysis. A reverse-phase column {C18} was used, with a {UV} detector at 214?nm and 0.05?M NaH2PO4 (pH 3.0)/acetonitrile (85:15, v/v) as the mobile phase. The accuracy of the analytical method was estimated by spiking bovine liver samples with three different concentrations of {CLB} (5.24?ng/g, 20.98?ng/g, and 41.96?ng/g) and recovery of 111.7%, 82.0%, and 84.8%, respectively, was obtained. The precision of the method was estimated by the relative standard deviation, which was??4.74%. The limit of detection and quantification of {CLB} were 0.20?ng/g and 0.42?ng/g of liver sample, respectively, and the retention time was 24.82 minutes. The recent discovery of {CLB} contamination in Mexican food led to the specific inspection of a distribution center for this β-agonist, involving the analysis of a total of 78 bovine liver samples. Of all samples screened, 62% of them had concentrations above the maximum residue limit of 0.6?ng/g set by the United Nations Food and Agricultural Organization for CLB. The analytical method was found to be rapid, sensitive, accurate, repeatable, and reproducible, and could be applied to the measurement of {CLB} concentration in bovine liver.