Comparative Evaluation of Antioxidant Potential of Parasitic Plant Collected from Different Hosts

摘要

Extracts of Cuscuta stem collected over different hosts were prepared by stimulated effect of ultrasonic waves using different solvents like 100% methanol, 80% methanol, 100% ethanol, 80% ethanol, water and n-hexane. Maximum extract yield (g/100 g of DW) was detected by using 80% methanol solvent (11.72-26.14) followed by water (10.52-22.95), 80% ethanol (10.19-22.63), methanol (8.91-22.61), ethanol (5.92-15.12), and n-hexane (3.82-8.72). Maximum level of TPC (71.11), TFC (85.11), reducing power (2.56), DPPH scavenging activity (59.57), percent inhibition of linoleic acid peroxidation (87.49) and 5-tocopherol (100 yg of 5 -Toc/g of FW) was found significantly higher (P < 0.05) in stem collected from Z. jojoba whereas a-(21 ug of 5 -Toc/g of FW) and Y-tocopherol (96 yg of 5 -Toc/g of FW) contents were found higher (P < 0.05) in E. jambulana and C. latifolia. A strong correlation between total phenolics (R,=0.916) or reducing power (R,=0.561) and antioxidant activity was found in methanolic extract whereas flavonoids exhibited weak correlation with antioxidant activity.