Constituents of Limonia acidissima inhibit LPS-induced nitric oxide production in BV-2 microglia

摘要

The ethyl acetate (EtOAc) soluble fraction of the 85% ethanol (EtOH) extract of the dried bark of Limonia acidissima potently inhibited nitric oxide (NO) production in lipopolysaccharide (LPS) activated BV-2 cells, a microglial cell line. Bioassay-guided column chromatography separation afforded a new stereoisomer of neolignan, (7’ E )-(7 R ,8 S )-4-hydroxy-3,5’-dimethoxy-4’,7-epoxy-8,3’-neolig-7’-en-9,9’-diyil diacetate ( 1 ), together with two known lignans, (+)-yangambin ( 2 ) and (+)-syringaresinol ( 3 ), three known triterpenoids, hederatriol ( 4 ), basic acid methyl ester ( 5 ), and 3β-hydroxyolean-12-en-11-one ( 6 ), and four known fatty acid derivatives, cascarillic acid ( 7 ), (+)-α-dimorphecolic acid ( 8 ), 8( R )-hydroxylinoleic acid ( 9 ), and (6 Z ,9 Z ,12 Z )-pentadecatrienoic acid ( 10 ). The structure of the new compound 1 was elucidated by detailed analysis of spectroscopic data and circular dichroism (CD) spectroscopy. Compounds 1 , 3-6 , and 8-10 isolated from L. acidissima significantly reduced NO production in LPS-stimulated BV-2 microglia cells.