PHENOLIC, FLAVONOID AND TANNIN CONTENT DETERMINATIONS AND IN-VITRO ANTI-OXIDANT ACTIVITY OF ROOT EXTRACTS OF SACCHARUM MUNJA ROXB.

摘要

Free radicals are reactive molecules involved in many physiological processes and have been associated with many diseases, such as ageing, cancer, arthritis, liver injury and cardiac complications. The fact between anti-oxidant potential and amount of polyphenolics compounds of Saccharum munja is very crucial co-relation because of its ehnomedicnal uses and experimental values. The total phenolics content were found to be 10.15?±2.45 mg gallic acid equivalent (GAE)/g total flavonoids contents and total tannin contents were 11.34?±1.20 mg RE/g and 14.54?±0.52 mg GAE/g respectively of Saccharum munja. The ability of Saccharum munja root extract was found to inhibit reactive oxygen species (ROS) free radicals. In the present study, the relative antioxidant ability of Saccharum munja was investigated through two in-vitro models, such as antioxidant capacity by radical scavenging activity using, ?±, ?±-diphenyl-?2-picrylhydrazyl (DPPH) and nitric oxide (NO) methods. The extracts were used at concentration 20, 40, 60, 80 and 100???μg/ml concentrations and radical scavenging activity was determined in terms of inhibition percentage. The IC50 (concentration required for 50% inhibition) were calculated for each radicals. The In-vitro free radical DPPH activities was found to 73.45 ?± 0.25 and NO antioxidant activity were found to be 57.20 ?± 1.15 at maximum concentration of 100?μg/ml. This study proven the significant role of hydro-alcoholic extract of Saccharum munja Roxb. as a potential source of natural antioxidants.