A Rapid and Effective Method for Simultaneous Determination of Residual Sulfonamides and Sarafloxacin in Pork and Chicken Muscle by High Performance Liquid Chromatography with Accelerated Solvent Extraction?Solid Phase Extraction Cleanup

摘要

A rapid and effective high performance liquid chromatographic (HPLC) method with accelerated solvent extraction (ASE)-solid phase extraction cleanup was developed for the determination of sulfonamide and sarafloxacin residues in pork and chicken muscle. Four residues were extracted with acetic acid-acetonitrile at 70°C under 10.3 MPa pressure and 5 min static times with 2 static cycles. The standard curves for the determination of four residues have good linearity (r>0.999). The method limits of quantification (LOQs) were 4-12 μg.kg-1. Intra- and inter-day precisions (RSDs) were 0.3-1.5% and 0.7-1.8%, respectively. Their average recoveries with four spiked levels ranged from 83% to 108% with the RSD of 0.3-1.5%. This method provides an effective extraction procedure and high sensitivity, and can be applied for the determination of 4 residual drugs in pork and chicken muscle at lower than their MRL levels.