The expression of Heparanase mRNA and its clinical significance in Hepatocellular carcinoma

摘要

Objective: To investigate the clinico-pathologic importance of Heparanase mRNA expression in hepatocellular carcinoma and its relationship with prognosis. Materials and Methods: Fresh tumors and noncancerous liver tissues were obtained from 30 hepatocellular carcinoma patients after hepatectomy. Expression levels of Heparanase and glyceraldehyde-3-phosphate dehydrogenase mRNA were quantitatively analyzed by real-time reverse transcriptase-polymerase chain reaction. Results: The relative Heparanase mRNA expression level (Heparanase: glyceraldehyde-3-phosphate dehydrogenase ratio) in hepatocellular carcinoma was lower than that in noncancerous tissue (P 0.05). Conclusion: Reduced Heparanase mRNA expression might result in abnormal cell growth and correlate with hepatocellular carcinoma progression.