The MOSAIC Standards Challenge: Capturing the Extent and Sources of Variability in NGS-Based Microbiome Profiling Protocols and Analyses

摘要

Several studies have documented the significant impact of the multitude of methodological variables in microbiome research and the effects on variability and overall consequences of NGS-based microbiome data. In most cases, these variables impede reproducibility of results and the ability of researchers to perform comparisons between independent studies that use differing techniques and data pipelines. To tackle this issue, the MOSAIC Standards Challenge will provide and ship 700 sets of fecal aliquots and DNA reference material, at no cost to Challenge participants, to any lab in the world. Each lab will perform their preferred sequencing protocols and upload their sequencing and metadata data to Mosaic. Data will allow to comparisons of the different protocols and analysis methods for taxonomic profiles. Articles from Journal of Biomolecular Techniques : JBT are provided here courtesy of The Association of Biomolecular Resource Facilities.