The MOSAIC Standards Challenge: Capturing the Extent and Sources of Variability in NGS-Based Microbiome Profiling Protocols and Analyses

摘要

Several studies have documented the significant impact of the multitude of methodological variables in microbiome research and the effects on variability and overall consequences of NGS-based microbiome data. In most cases, these variables impede reproducibility of results and the ability of researchers to perform comparisons between independent studies that use differing techniques and data pipelines.