Genetic Diversity Analysis among Accessions of Desmodium gangeticum (L) DL with Simple Sequence Repeat (SSR) and Internal Transcribed Spacer (ITS) Regions for Species Conservation

摘要

Desmodium gangeticum is one of the important species of ethno- medicinal plant. It finds its uses in numerous medicinal preparations including many industrial applications. Inspite of an important medicinal plant, till date little work have been conducted towards its molecular characterization and conservation. Therefore the present study was designed to bring out the relationships among different available accessions and study biodiversity conservation through simple sequence repeat (SSR) and inter transcribed spacer markers. The analysis revealed that intrapopulation mean genetic value (1.835) and Shannon (0.641) values vary among the accessions. However through AMOVA analysis, we observed that only a small variation exists among these accessions (P=1). The dendogram generated through distance matrix using the SIMQUAL-Dice Coefficient module of NTSYS pc ver matches to some extent with the PCA graph. In the barcode ITS marker analysis UPGMA shows the clustering of accessions into two groups. It is thus concluded that above based markers can be used for the population conservation and authentication of the above plant and could also be a lead for other medicinally important plants.