Optimization of Light intensity, Nitrate concentration and Cultivation time for Biomassproduction by Chlorella minutissima using Response Surface Methodology

摘要

Chlorella minutissima was investigated for biomass production under different growth conditions. Box Behnken design was employed to study the effect of different growth variables. Three variables light intensity, nitrate concentration and cultivation time were identified as having significant effects and interactions during batch cultivation of the alga in a stirred tank bioreactor. Using the experimental data a second order regression model was developed. It was found that the biomass production by Chlorella minutissima was strongly affected by the variations in light intensity, nitrate concentration and cultivation time. The experimental values were in good agreement with the predicted values, correlation coefficient was found to be 0.9212. The optimized conditions for the enhancement of the biomass production by C. minutissima were determined to be light intensity (8153.85 lx), nitrate concentration (2.5 g/l) and cultivation time (9 days).