Cellular expression of CD54 and estimation of sIL-2R, sVCAM-1 and sE-selectin inChildren with Acute Lymphoblastic Leukemia

摘要

Measurement of some circulating endothelial adhesion molecules levels and interleukin receptors have been suggested as additional tools for assessment of patients with acute lymphoblastic leukemia (ALL). The aim of this study is to estimate circulating sVCAM-1. sE-selectin and sIL2-R in the serum of patients with acute lymphoblastic leukemia before and after chemotherapy and comparing them to the control group. Also to detect the cellular expression of CD 54 on bone marrow cells of children with acute lymphoblastic leukemia and comparing its level in responders and nonresponders to treatment.This study included seventy subjects attending National Cancer Institute; forty newly diagnosed patients with acute lymphoblastic leukemia (ALL), 10 patients in relapse and 20 apparently normal subjects within the same age and sex range, as a control group. Acute lymphoblastic leukemia diagnosis was made by bone marrow aspiration, cytochemistry and microscopic examination. Flowcytomer was used for immunophenotyping to confirm the diagnosis using monoclonal antibodies. Expression of CD54 on mononuclear cells is detected by monoclonal antibodies using flow cytometry. Soluble sIL2-R, sVCAM-1 and sE-selectin were estimated using enzyme-linked immunosorbent assay.There were significant increases in the estimated parameters in patients before chemotherapy as compared to after treatment as well as to the control group. Initial CD54 cellular expression was significantly higher in responders as compared to nonresponders to treatment. In conclusion, the levels of some soluble circulating adhesion molecule levels can be utilized for monitoring the disease activity of ALL and its response to treatment. Also the initial low cellular expression of CD54 is a predictor for adverse prognosis.