The Effect of Photodynamic Therapy on Microbial Reduction in Diabetic Rats withInduced Periodontitis

摘要

Aim: to compare the outcome of conventional mechanical therapy with or without adjunctive photodynamic therapy (a PDT) on the number of Porphyromonas gingivalis (P.g.) and Aggregatibacter actinomycetemcomitans (A.a.) bacteria in experimentally induced periodontitis in diabetic and non-diabetic rats. Materials and methods: The animals were divided randomly into 2 groups of 30 rats each: In the non-diabetic group, only experimental periodontitis was induced, while in the diabetic group, both experimental periodontitis and diabetes were induced. Both diabetic and non-diabetic groups were further divided into two subgroups with 15 rats in each subgroup. In the non-diabetic group 15 randomly chosen rats were treated with SRP in addition to PDT, while the other 15 rats received only SRP treatment. The same treatment protocol was carried in the diabetic group. Results: The use of aPDT resulted in statistically significantly lower mean number of P.g. and A.a. in the non-diabetic groups than SRP alone through all time intervals. In the diabetic animals, the use of a PDT resulted insignificantly lower mean number of P.g. than SRP alone only after 1 and 3 months, while in case of A.a., this treatment resulted in significantly lower mean number of A.a. than SRP alone through all time intervals. Conclusion: Both SRP and SRP +PDT decreased the number of pathogens in both groups, however results showed a PDT demonstrated significant lower mean numbers of P.g. and A.a.than SRP alone. This effect was more pronounced in the non- diabetic than the diabetic animals.