Objective To establish Zucker diabetic fatty rats with periodontitis model, to explore the effect of periodontitis on the severity of diabetes-related complications in models, and to analyze its mechanism.Methods 48 female Zucker diabetic fatty (ZDF, fa/fa) rats were included and randomly divided into Group A and B, each group further randomized into Group 1 and 2.Group A was made model of periodontitis, in which Group A1 was given high fat diet, in order to induce insulin resistance (IR), while Group A2 was given low fat diet.Group B was not made model of periodontitis, in which Group B1 was given high fat diet, while Group A2 was given low fat diet.Glucose tolerance test was carried out, fasting insulin and blood glucose were measured every week.13 weeks later, the rats were sacrificed, and the health condition of periodontal tissue was detected, in order to evaluate the reliability of modeling.Serum TNF-α, IL-1β, CRP and pathological changes in aorta, fundus, pancreatic tissues were detected and analyzed statistically.Results ① The modeling scheme is reliable.Fasting blood glucose, fasting insulin, and HOMA-IR in Group A1 and B1 were respectively higher than in Group A2 and B2, and the difference was statistically significant (P<0.05).There was obvious periodontal inflammation, periodontal soft tissue destruction, and bone resorption in Group A.② The serum TNF-α, IL-1β, CRP in Group A1 and B1 were respectively higher than in Group A2 and B2, and the difference was statistically significant (P<0.05).There was a positive correlation between the above indexes and HOMA-IR (P<0.05).③ The pathological changes of aorta, fundus and pancreatic tissue were significantly higher in A1 group than in other groups, and the difference was statistically significant (P<0.05).Conclusion Periodontitis can lead to the increase of IR degree and the severity of diabetes-related complications in Zucker diabetic fatty rats, which may be related to the increase of serum inflammatory factors in rats with periodontitis.%目的 建立牙周炎Zucker糖尿病肥胖大鼠模型,探讨牙周炎对模型糖尿病相关并发症严重性程度的影响,并分析其机制.方法 以雌性Zucker糖尿病肥胖(zucker diabetic fatty rats, ZDF,fa/fa)大鼠48只为实验材料,随机均分为A、B组,每组再随机均分为1、2组:A组牙周炎造模,A1组接受高脂饮食诱导胰岛素抵抗(insulin resistance, IR),A2组接受低脂饮食;B组无牙周炎造模,B1组接受高脂饮食,B2组接受低脂饮食.每周开展糖耐量实验,测量空腹胰岛素及血糖,13周后处死大鼠,检测牙周组织健康状况,以评价造模可靠性.检测血清肿瘤坏死因子-α(tumornecrosisfactor-a, TNF-α)、白细胞介素-1β(interleukin-1β, IL-1β)、C反应蛋白(C-reactive protein, CRP),观察主动脉、眼底、胰腺组织病理学变化,行统计学分析.结果 ①造模方案可靠,A1组、B1组空腹血糖、空腹胰岛素、IR指数(HOMA-IR)分别高于A2、B2组,差异有统计学意义(P<0.05);A组出现明显的牙周炎症,牙周软组织破坏、骨吸收明显.②血清TNF-α、CRP及IL-1β水平呈A1、B1组高于A2、B2组趋势,差异有统计学意义(P<0.05),且上述指标与IR指数呈正相关性(P<0.05).③各组主动脉、眼底、胰腺组织病变程度,A1组明显高于其他各组,差异有统计学意义(P<0.05).结论 牙周炎可导致糖尿病肥胖大鼠IR程度上升、加剧其糖尿病相关并发症严重程度,这可能与牙周炎可导致大鼠血清炎性因子水平上升有关.
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