Extraction and Fractionation of Whole Black Seed Plantain ( Plantago rugelii Decne) for in-vitro Antioxidant, Antibacterial and Phytochemical Screening

摘要

This study was designed to explore new antioxidant and antimicrobial agents from the methanol wholeplant crude extract and fractions of Plantago rugelii. The methanol extract and its fractions were prepared and screenedfor its phytochemical composition, in-vitro antioxidant potential and challenged with common pathogenic microorganismsfor its antimicrobial activities using standard procedures. The phytochemical analysis revealed the presence of variouspharmaceutically active secondary metabolites like alkaloids, phenolic, flavonoids, carbohydrates, glycosides, sterols, etc.In the DPPH assay, the aqueous methanol fraction was found to be the most effective among all the fractions in comparisonto the ascorbic acid standard. Using the zone of inhibition as inhibitory parameter, the crude methanol extract exhibitedthe best antibacterial activity when challenged against all the clinical isolates except Klebsiella pneumoniae . The aqueousmethanol extract exhibited the best percentage fungal inhibition when compared to other fractions. The standard drugsciprofloxacin and fluconazole exhibited a near 100% activity except with Proteus vulgaris where the crude methanolextract has a higher value.This research holds promise for the exploration of various potentially active secondarymetabolites which would help in developing pharmaceuticals, especially antioxidant and antimicrobial drugs. Theisolation and characterization of the exact metabolites responsible for these activities is therefore recommended.