Purification and Analysis of Norcadia spp .Azoreductase

摘要

Nocardia spp. was isolated as Acid Red degrader. The reductive cleavage of azo bond was catalyzed by azo reductase, the key enzyme for the azo dye degradation. Azo reductase from the Nocardia spp. isolate was purified and characterized. Azoreductase was found to have the following features. The catalytic reduction of acid red 37 by purified azoreductase in the presence of NADH as electron donor was studied and the products of degradation were determined as 1-{3-amino-5-[(aminoxy)sulfonyl]phenyl}ethanol and 7,8-diamino-3[(aminoxy)sulfonyl]naphthalene-1-ol.