Evaluation of PFKM, TFDP2, and HIP2 Gene Expression and Muscle Growth in Sheep

摘要

Genes involved in metabolic pathways contributing to hormone-induced muscle hypertrophy are possible markers that can be used to select for increased muscle growth. Phosphofructokinase muscle-type, transcription factor dp-two, and huntingtin-interacting protein are three candidate genes which are known to be involved in metabolic pathways controlling energy metabolism and the cell cycle. The objective of this study was to analyze the effect of testosterone on the mRNA abundance from these three genes in splenius and semitendinosus muscles in rams and wethers at four different age points. Analysis of gene expression showed no differences in mRNA abundance between semitendinosus and splenius muscles in both rams and wethers at four different age groups for the three genes analyzed. Further expression analysis is needed to determine the contribution of other candidate genes to controlling hypertrophy in sexually dimorphic muscles.