Study on Bovine Mammary Specific Expression Vector of Expressing Human Lysozyme Gene

摘要

To study the bovine mammary gland bioreactor, the 5' and 3' flanking sequence of bovine β-casein (CSN2) gene were cloned and connected to the eukaryotic expression vector of pC DNA3.0 which had been modified. The human Lysozyme gene (hLYZ) was inserted into the site between 5' and 3' flanking sequence after the vector named pC was identified and formed the vector named as pC-hLYZ subsequently. To determine whether the constructed vector could drive the hLYZ expression, the pC-hLYZ and positive control hLYZ plasmid were wrapped by the PEI and injected into lactation rabbits through mammary gland centre duct. Milk was collected and the expression status was detected after 72 h. The plate inhibition assay showed that pC-hLYZ eukaryotic expression vector could be a good driver for hLYZ gene expression in rabbit mammary gland. The results demonstrated that the bovine mammary gland specific expression vector of hLYZ had been constructed successfully. The establishment of the vector laid the foundation for further study of bovine mammary gland expression technology.