Detection of sef14, sef17 and sef21 Fimbrial Virulence Genes of Salmonella enteritidis by Multiplex PCR

摘要

Salmonella serovars show their diverse effects by joining the gastrointestinal epithelia. The infectious trait of this bacterium depends on colonization in GI epithelial surfaces. Fimbirae play the main role in this junction. Three kinds of fimbrial genes are more important and responsible for the pathogenecity and the attachment of Salmonella enteritidis to intestinal epithelium (sef14, 17, 21). Therefore, researchers have attempted to study the significance of fimbriae genes by the molecular method. In first of all, the molecular method has to be optimized for investigating the presence of these genes in the confirmed isolates of S. enteritidis. About 45 isolates of S. enteritidis collected from 60 diarrheic feces of both livestock and avian population with clinical sign of diarrhea were considered. In order to trace the most important fimbrial genes, the multiplex PCR method was designed and optimized. The optimal primers, annealing temperature of primers and concentration of DNA template, MgCl2 and Taq DNA polymerase were determined.