首页> 外国专利> MULTIPLEX PCR KITS AND PRIMERS OF THE DIFFERENTIATION AND SIMULTANEOUS DETECTION FOR 3 SEROVARS OF CHOLERAESUIS, ENTERITIDIS, TYPHIMURIUM IN SALMONELLA

MULTIPLEX PCR KITS AND PRIMERS OF THE DIFFERENTIATION AND SIMULTANEOUS DETECTION FOR 3 SEROVARS OF CHOLERAESUIS, ENTERITIDIS, TYPHIMURIUM IN SALMONELLA

机译:多重PCR试剂盒和沙门氏菌3种猪霍乱弧菌,肠炎,鼠伤寒血清的鉴别和同时检测

摘要

The present invention relates to a primer set for detecting salmonella common genes and screening three salmonella serotypes, a multiplex polymerase chain reaction kit including the primer set, and a method for detecting salmonella common genes and screening three salmonella serotypes using the kit, and more particularly, to: (1) a primer set for detecting salmonella common genes comprising a forward primer of sequence number 1 and a reverse primer of sequence number 2; (2) a primer set for detecting S. Typhimurium comprising a forward primer of sequence number 3 and a reverse primer of sequence number 4; (3) a primer set for detecting S. Choleraesuis comprising a forward primer of sequence number 5 and a reverse primer of sequence number 6; (4) a primer set for detecting S. Choleraesuis comprising a forward primer of sequence number 7 and a reverse primer of sequence number 8; a multiplex polymerase chain reaction kit including a primer set from (1) to (4); and a method for detecting salmonella common genes and screening three salmonella serotypes using the kit.
机译:本发明涉及一种用于检测沙门氏菌共有基因并筛选三种沙门氏菌血清型的引物组,包括该引物组的多重聚合酶链反应试剂盒,以及用于检测沙门氏菌共有基因并使用该试剂盒筛选三种沙门氏菌血清型的方法。 (1)一种用于检测沙门氏菌共有基因的引物组,其包括序列号为1的正向引物和序列号为2的反向引物; (2)一种用于检测鼠伤寒沙门氏菌的引物组,其包括序列号为3的正向引物和序列号为4的反向引物。 (3)用于检测霍乱链球菌的引物组,其包含序列号5的正向引物和序列号6的反向引物。 (4)用于检测霍乱链球菌的引物组,其包含序列号为7的正向引物和序列号为8的反向引物。多重聚合酶链反应试剂盒,其包括从(1)至(4)的引物组;一种检测沙门氏菌共有基因并使用该试剂盒筛选三种沙门氏菌血清型的方法。

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