Identification of One Putative D7 Related Gene Specifically Expressed in Female Anopheles anthropophagus

摘要

Anopheles anthropophagus is an important mosquito vector transmitting malaria and Brugia malayi. Due to differences in feeding behaviors between the female and male adult An. anthropophagus, female An. anthropophagus plays a more important role in transmitting pathogens than male ones. The present study identified a gene specifically expressed in female An. anthropophagus from a subtractive cDNA library for female An. anthropophagus and its full-length cDNA was amplified by 3' and 5' Rapid Amplification of cDNA Ends (RACE) technique. This gene was named fsyz and further analyzed by using Northern blot and bio-informatics analyses. It was shown that the fsyz could encode a D7 related salivary protein and was female-specifically expressed and not characterized previously worth further studies.